Does the MultiPep RSi have to be operated in a fume hood? No, the instrument comes with a self-contained cabinet. An exhaust tube leading to a ventilation system must be connected to an outlet provided.
How easy is a change of synthesis scale on the MultiPep RSi? To select a different scale you have to load the correct synthesis plate and select a protocol corresponding to this scale. We supply optimized routines for several synthesis scales.
Is there any synthesis monitoring in the MultiPep RSi? No, the MultiPep RSi follows an optimized coupling protocol. Sequence-related problems must be deducted from the mass spectrum of the final peptide product.
Can I use bulk reagents? Yes, the MultiPep RSi was designed for economical operation. Solvents and activator are bought from standard, recommended suppliers. Only amounts needed for a particular synthesis are weighed, dissolved and used.
Which reaction plates are delivered with the MultiPep RSi? Our default scale is 5 µmol and we will deliver a basic set of 96 well reaction plates and amino acid vials for this scale. The plates are also usable at a scale of 1 µmol, and we provide a different synthesis plate to be used for a scale of 5-10 µmoles.
How do we fill empty synthesis plates? To fill the individual cavities of the reaction plate you make a slurry of resin and then pipet it into the wells.